ANTIOXIDANT ACTIVITY TEST OF TURI (Sesbania grandiflora L.) LEAF EXTRACT USING β-CAROTENE BLEACHING METHOD

Salsabilah Lutfiah

Salsabilah Lutfiah Universitas Muslim Indonesia

Keywords: Turi Leaves (Sesbania grandiflora L.), Antioxidant, β-carotene bleaching, UV-Vis spectrophotometry

Abstract

Turi Leaf (Sesbania grandiflora L.) belong to the fabaceae family that contain flavonoids as an antioxidants. The antioxidants have free radical scavenging properties that can inhibit the oxidation of free radicals. It is used to protect the skin from oxidative damage and to prevent premature aging. This research aimed to determine the antioxidant activity of turi leaf extract by b-carotene bleaching method. The leaves were extracted using macerations method, the yield percentage of 16,685 %. Quantitative analysis by UV-Vis spectrophotometry at the maximum wavelength of 538 nm. The results showed that the antioxidant activity of the turi leaf extract is 50.7% which is intermediate antioxidant, then in quercetin standard solution.

Keywords : Turi Leaves (Sesbania grandiflora L.), Antioxidant, β-carotene bleaching, UV-Vis spectrophotometry

References

[1] Handayani, S., Najib, A., & Wati, N. P. (2018). Uji Aktivitas Antioksidan Ekstrak Daun Daruju (Acanthus ilicifolius L.) Dengan Metode Peredaman Radikal Bebas 1,1-Diphenyil-2-Picrylhidrazil (DPPH). Jurnal Fitofarmaka Indonesia, 5(2), 299–308.
[2] Hasanah, N. (2015). Aktivitas Antioksidan Ekstrak Etanol Daun Salam. Jurnal Pena Medika, 5(1), 55–59.
[3] Binuni, R., Maarisit, W., Hariyadi, H., & Saroinsong, Y. (2020). Uji Aktivitas Antioksidan Ekstrak Daun Mangrove (Sonneratia alba) Dari Kecamatan Tagulandang, Sulawesi Utara Menggunakan Metode DPPH. Biofarmasetikal Tropis, 3(1), 79–85.
[4] Werdhasari, A. (2014). Peran Antioksidan Bagi Kesehatan. Jurnal Biomedik Medisiana Indonesia, 3(2), 59–68.
[5] Tahir, M., Abidin, Z., & Sukmawati, N. (2017). Antioxidant activity of hydrolyzed black soybean (Glycine Soja Linn. Sieb.) by β-Carotene Bleaching. Journal of Pharmaceutical and Sciences, 2(1), 1–4.
[6] Rohmah, J., Rachmawati, N. R., & Nisak, S. (2018). Perbandingan Daya Antioksidan Ekstrak Aseton Daun dan Batang Turi Putih (Sesbania grandiflora) dengan Metode DPPH (diphenilpycrylhydrazil). Seminar Nasional Hasil Riset dan Pengabdian, 665–677.
[7] Gerald Masengi, J. M., Diah Puspawati, G. A. K., & Sri Wiadnyani, A. A. I. (2020). Pengaruh Jenis Pelarut Terhadap Aktivitas Antioksidan Ekstrak Cair Daun Turi (Sesbania grandiflora). Jurnal Ilmu dan Teknologi Pangan (ITEPA), 9(2), 242.
[8] Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia alba. Jurnal Perikanan Dan Kelautan Tropis, 11(1), 9–15.
[9] Shetty, K., Paliyath, G., Pometto, A., dan Levin, R, E., 2007, Functional Foods and Biotechnology, CRC. Press, USA
[10] Salamah, N., dan Nurushoimah, 2014, Uji Aktivitas Antioksidan Ekstrak Etanol Herba Pegagan (Centella asiatica (L.) Urb.) dengan Menggunakan Metode β-carotene, Jurnal Farmasi
[11] Yuhernita, Juniarti. Analisis Senyawa Metabolit Sekunder Dari Ekstrak Metanol Daun Surian Yang Berpotensi Sebagai Antioksidan. Makara Sains, 2014, 15 (1) : 1